Sheep anti Human Prothrombin polyclonal antibody

Sheep Anti-Human Prothrombin polyclonal antibody for IEP, ELISA. Prothrombin (factor II, F.II) is a vitamin K-dependent glycoprotein produced in the liver. The concentration of prothrombin in plasma is ~100 Вµg/ml (~1.4 ВµM). Prothrombin is a single chain molecule with a molecular weight of 72 kDa. Prothrombin consists of a catalytic domain followed by two kringle structures and an amino-terminal domain containing 10 ?-carboxy-glutamic acid (gla) residues. These gla residues allow prothrombin to bind to membranes that contain acidic phospholipids in a calcium dependent manner. The binding to membranes is required for effective presentation of prothrombin as a substrate for activation by the prothrombinase complex, which consists of activated factor X, activated cofactor V and calcium on phospholipid membrane. Activation by prothrombinase occurs by sequential cleavage after residue Arg320 then after Arg271 to produce the active protease ?-thrombin (37 kDa) and the byproduct prothrombin fragment 1.2 (35 kDa). The product thrombin further cleaves prothrombin fragment 1.2 after residue Arg155 into individual prothrombin fragments 1 and 2. The activity of ?-thrombin in plasma is inhibited primarily by antithrombin and the rate of inhibition is accelerated 1000-fold in the presence of optimal concentrations of heparin. Other physiological inhibitors of thrombin in the absence of heparin include ?2 macroglobulin and ?1 Antitrypsin.

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SPECIFICATIONS

Catalog Number

CABT-L402

Applications

ELISA

Hosts

Sheep

Reactivities

Hum

Conjugates

Unconj

Target

F2

Gene Id

2147

Isotype

IgG

Clonality

Polyclonal

Storage Temp

Store between -10 and -20В°C. Product will become viscous but will not freeze. Avoid storage in frost-free freezers. Keep vial tightly capped. Allow product to warm to room temperature and gently mix before use.