Sheep anti-IgA Secretory Piece Polyclonal antibody for RID, IEP, ELISA. Suitable for use in a variety of gel techniques including radial immunodiffusion (RID), double diffusion and immunoelectrophoresis (IEP). The use of 3% PEG 6000 with 1.2% agarose in a suitable buffer (such as TBE or Tris-barbital pH 8.2) is recommended. Suitability for use in immunohistochemical procedures, enzyme-linked immunosorbent assays and Western blot has not been assessed but use in such assays should not necessarily be excluded. The following conditions should be used only as guidelines.RID: 16ul antiserum/cm2 in gel vs 5ul fresh human milk, Neat - 1:5.DoubleDiffusion: 10ul antiserum vs 10ul fresh human milk. IEP: 100ul antiserum vs 10ul fresh human milk.