Sheep anti-Mouse IgE Polyclonal antibody for DID, IEP. This product is suitable for use in a variety of gel techniques including double diffusion and IEP. The use of3% PEG 6000 with 1.2% agarose in a suitable buffer (such as TBE or Tris-barbital pH 8.2) is recommended. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded. Double Diffusion: 10ul antiserum vs 10ul mouse IgE IEP: 100ul antiserum vs 5ul mouse IgEAll five immunoglobulin classes share the same basic four polypeptide chain structure of two heavy-chains and two light chains. There are five heavy chain types, and two light-chain types (Kappa and Lambda) both having a molecular weight of 22.5kDa. Any heavy-chain type can associate with either light-chain type, but on any immunoglobulin molecule both light-chains are of the same type. Kappa and Lambda consist of a variable region and a constant region and can easily be
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