Hedgehog (Hh) proteins are widely distributed throughout the animal kingdom and play important regulatory roles in various development processes. The three known mammalian Hh proteins, Sonic (Shh), Desert (Dhh) and Indian (Ihh) are structurally related and share a high degree of aa sequence identity (e.g. Shh and Ihh are 93 % identical). Although, Hh proteins have unique expression patterns and distinct biological roles within their respective regions of secretion, they signal through the same Patched (Ptc)/Smoothened (Smo) receptor complex, activate the same intracellular signaling pathway, and can substitute for each other in experimental systems. The mature biologically active form of Hh molecules is produced by autocatalytic cleavage of their precursor proteins and corresponds to approximately the N-terminal half of the precursor molecule. Recombinant human Shh is a 20.6kD protein consisting of 174aa, which correspond to the active N-terminal portion of the Shh precursor.
Source:
Recombinant protein corresponding to human SHH, expressed in (BTI-Tn-5B1-4) High-5 Insect. Cells.
Biological Activity:
Determined by its ability to induce alkaline phosphatase production by C3H/10T1/2 (CCL-226) cells. The expected ED50 for this effect is 0.8-1ug/ml.
Endotoxin:
<0.1ng/ug (1EU/ug)
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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