SHP 1, Recombinant, Human, aa243-541 (SHPTP-1, SH2 containing protein tyrosine phosphatase 1, PTP1C, SH-PP1, HCP, SHP, PTPN6)

The protein coding region of the catalytic domain of SHP-1 (amino acids 243-541). The catalytic domain of SHP-1 was overexpressed as insoluble protein aggregates (inclusion bodies). Recombinant SHP-1 protein was purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a redox buffer. Additional amino acid(Met) is attached at N-terminus. Recombinant SHP-1 produced in E.Coli is a single,non-glycosylated polypeptide chain conjtaining 300 amino acids and having a molecular mass of 34.3kD.\n\nSequence: \nMGFWEEFES LQKQEVKNLH QRLEGQRPEN KGKNRYKNIL PFDHSRVILQ GRDSNIPGSDYINANYIKNQ LLGPDENAKT YIASQGCLEA TVNDFWQMAW QENSRVIVMT TREVEKGRNKCVPYWPEVGM QRAYGPYSVT NCGEHDTTEY KLRTLQVSPL DNGDLIREIW HYQYLSWPDHGVPSEPGGVL SFLDQINQRQ ESLPHAGPII VHCSAGIGRT GTIIVIDMLM ENISTKGLDCDIDIQKTIQM VRAQRSGMVQ TEAQYKFIYV AIAQFIETTK KKLEVLQSQK GQESEYGNITY\n\nActivity: \n~5000 U/mg\n\nUnit Defenition: \nOne unit will hydrolyze 1nanomole of p-nitrophenylphosphatate per minute at pH 7.5 at 37 degrees C using 10mM of substrate.\n\nStability: \nStore at 4 degrees C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degrees C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

S1013-25B

Size

5ug

Form

Supplied as a liquid in 25mM Tris-HCl, pH 7.5, 2mM beta-mercaptoethanol, 1mM EDTA, 1mM DTT and 20% glycerol.

Purity

(same/more than) 95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.

References

1. Inverse regulation of inducible nitric oxide synthase (iNOS) and arginase I by the protein tyrosine phosphatase SHP-1 in CNS glia.\n\nGlia 2006 Jun;53(8):827-35\n\n2. Deficiency of SHP-1 protein-tyrosine phosphatase in \" viable motheaten \" mice results in retinal degeneration.\n\nInvest Ophthalmol Vis Sci 2006 Mar;47(3):1201-9\n\n3. Impairment of SHP-1 down-regulation in the lipid rafts of human neutrophils under GM-CSF stimulation contributes to their age-related, altered functions.\n\nJ Leukoc Biol 2006 May;79(5):1061-72\n\n4. SHP-1 inhibits LPS-mediated TNF and iNOS production in murine macrophages.\n\nBiochem Biophys Res Commun 2006 Apr 7;342(2):547-55\n\n5. Shp-1 mediates the antiproliferative activity of tissue inhibitor of metalloproteinase-2 in human microvascular endothelial cells.\n\nJ Biol Chem 2006 Feb 10;281(6):3711-21\n\n6. Decoding protein-protein interactions through combinatorial chemistry: sequence specificity of SHP-1, SHP-2, and SHIP SH2 domains.\n\nBiochemistry 2005 Nov 15;44(45):14932-47