Siglec-1 (Sialic Acid Binding Ig-like Lectin 1, CD169, CD169 Antigen, dJ1009E24.1, DKFZp667F058, FLJ00051, FLJ00055, FLJ00073, FLJ00411, FLJ32150, Sialic Acid-binding Ig-like Lectin 1, Sialic Acid-binding Ig-like Lectin-1, Sialoadhesin, Sialoadhesin Precu

CD169, otherwise known as Sialoadhesin (Sn), is a type I transmembrane glycoprotein and prototypic member of the Siglec (sialic acid binding Ig-like lectin) family, designated Siglec-1.CD169 is a macrophage-restricted interaction molecule which binds to sialylated ligands on haematopoietic cells, including neutrophils, NK cells, monocytes, a subset of CD8+ T cells and B cells, through recognition of sialic acid in the alpha-2,3- glycosidic linkage. CD169 is highly expressed by dendritic cells and on stromal macrophages in the spleen, bone marrow and lymph nodes and to a lesser extent in liver, lungs and gut, and studies have shown high expression of CD169 on macrophages in the inflammatory conditions rheumatoid arthritis and atherosclerosis. Completely inhibits erythrocyte rosetting around Sn+ transductants. \n\nApplications: \nSuitable for use in Flow Cytometry. Other applications not tested.\n\nRecommended Dilutions:\nFlow Cytometry: Neat-1:10 Use 10ul to label 1x10e6 cells in 100ul.\nOptimal dilutions to be determined by the researcher.\n\nHybridoma: \nSpleen cells from immunized Balb/c mice were fused with cells of the X63-Ag8.653 myeloma cell line.\n \nRecommended Negative Control:\nI1904-79D:IgG1 Murine Negative Control (PE)\n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C. Reconstitute by adding sterile ddH2O. Aliquot and store at 4 degrees C. DO NOT FREEZE! Reconstituted product is stable for 12 months as an undiluted liquid. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S1013-57

Size

100Tests

Applications

FC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a lyophilized powder from PBS, pH 7.4, 1% BSA, 0.09% sodium azide. Labeled with PE. Reconstitute with 1ml sterile ddH2O.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Kirchberger, S. et al. (2005) Human rhinoviruses inhibit the accessory function of dendritic cells by inducing sialoadhesin and B7-H1 expression. J. Immunol. 175: 1145-1152. 2. Hartnell, A. et al. (2001) Characterization of human sialoadhesin, a sialic acid binding receptor expressed by resident and inflammatory macrophage populations. Blood. 97: 288-296.

Additional Info

Recognizes human Siglec-1. Completely inhibits erythrocyte rosetting around Sn+ transductants.