sIL-2Ra, Recombinant, Human (soluble IL-2 receptor, TAC-antigen, CD25 antigen)

The IL-2 receptor system consists of three non-covalently linked subunits termed IL-2Ralpha, IL-2Rbeta, and IL-2Rgamma.The IL-2Ralpha is a type I transmembrane protein consisting of a 219 amino acid extracellular domain, a 19 amino acid transmembrane domain and a 13 amino acid intracellular domain, which is not involved in the transduction of IL-2 signals.Proteolytic processing of IL-2Ralpha releases the entire extracellular domain of IL-2Ralpha thereby generating a 219 amino acid soluble protein called soluble IL-2Ralpha (sIL-2Ralpha).The homodimeric form binds IL-2 (KD=10nM) and facilitates IL-2 signaling.The secreted sIL-2Ralpha is expressed on leukemia cells, lymphoma cells, newly activated T and B cells, as well as on approximately 10% of NK cells.Recombinant human sIL-2Ralpha is a 24.8kD protein containing 219 amino acid residues consisting of only the extracellular domain of IL-2Ralpha. Due to glycosylation, IL-2Ralpha has an approximate molecular weight of 31kD based on SDS-PAGE gel and Mass Spectrometry.* Manufactured using (BTI-Tn-5B1-4) cells under license from the Boyce Thompson Institute for Plant Research, Inc.\n\nBiological Activity:\nDetermined by its ability to increase the proliferation effect of IL-2 in murine CTLL-2 cells. In the presence of 1ng/ml of recombinant IL-2, the expected ED50 for this effect is between 0.5-1.5ug/ml.\n\nAA Sequence:\nELCDDDPPEI PHATFKAMAY KEGTMLNCEC KRGFRRIKSG SLYMLCTGNS SHSSWDNQCQ CTSSATRNTT KQVTPQPEEQ KERKTTEMQS PMQPVDQASL PGHCREPPPW ENEATERIYH FVVGQMVYYQ CVQGYRALHR GPAESVCKMT HGKTRWTQPQ LICTGEMETS QFPGEEKPQA SPEGRPESET SCLVTTTDFQ IQTEMAATME TSIFTTEYQ\n\nCrossreactivity:\nMouse\n\nQuality Control:\nVerified by N-terminal and Mass Spectrometry analyses (when applicable).\n\nEndotoxin:\n<0.1ng/ug of protein (<1EU/ug).\n\nProtein Content:\nVerified by UV Spectroscopy and/or SDS-PAGE gel.\n\nStorage and Stability:\nStore lyophilized products at -20 degrees C. For reconstituted solutions of most products, we recommend short-term storage at 4 degrees C. For longer term storage the protein solution should be stored with a carrier protein (eg. 0.1% BSA) in working aliquots and stored frozen at -20 degrees C. Additional freeze/thaw cycles may cause some denaturation of the protein.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts