SIRP-(alpha)1 (R479) pAb

SIRPs (signal-regulatory proteins) are a family of transmembrane glycoproteins that were identified by their association with the Src homology 2 domaincontaining protein-tyrosine phosphatase SHP-2 in response to insulin. The SIRP family negatively regulates the PI 3-K pathway, which may diminish EGFR-mediated motility and survival phenotypes that contribute to transformation of certain cell types. SIRP-(alpha)1 is a transmembrane protein which contains an extracellular portion with three immunoglobulin-like structures and a cytoplasmic region with four potential tyrosine phosphorylation sites. SIRP-(alpha)1 is a substrate for activated receptor tyrosine kinases. In its tyrosine phosphorylated form, SIRP-(alpha)1 binds to SH-PTP2 through SH2 interactions and acts as an SH-PTP2 substrate. SIRP-(alpha)1 has been shown to have negative regulatory effects on cellular responses induced by growth factors, oncogenes and insulin. SIRP-(beta)1 shares extensive sequence homology with SIRP-(alpha)1 in its extracellular portion but lacks the cytoplasmic portion. SIRP-(gamma), originally designated SIRP-(beta)2 (SIRP-B2, CD172g) has unique characteristics from both the (alpha) and (beta) versions.

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SPECIFICATIONS

Catalog Number

BS1324

Size

100ug/100ul

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

liquid

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Antigen

Synthetic peptide, corresponding to amino acids 450-500 of Human SIRP-(alpha)1.

Species

Sirp (alpha)1 (R479) pAb detects endogenous levels of Sirp (alpha)1 protein.

Dilutions

WB: 1:500~1:1000\nIHC: 1:50~1:200

Swiss Prot

P78324

Storage Temp

Store at 4 degrees C short term. Aliquot and store at -20 degrees C long term. Avoid freeze-thaw cycles.

Concentration

1ug/ul