SIRP, alpha1, aa487-503, Blocking Peptide (SIRPa, Signal Regulatory Protein, SHPS-1, BIT, p84, CD172a)

Control peptide for S1013-87.\n\nProtein tyrosine phosphatases (PTPases) SHP-1 and SHP-2 are critical regulators in the intracellular signaling pathways that result in cell responses such as mitosis, differentiation, migration, survival, transformation or death. SHP-2 is a signal transducer for several receptor tyrosine kinases and cytokine receptors. A novel SHP-2 associated glycoprotein was recently cloned from human, rat, mouse and cattle by several labs and was designated SIRPa, SHPS-1, MyD-1, BIT and p84. SIRPa is a new gene family containing at least fifteen members. SIRPa is a substrate of many activated tyrosine kinases such as insulin receptor, EGFR, PDGFR and src, and a specific docking protein for SHP-2. SIRPa has regulatory effects on cellular responses induced by serum, growth factors, insulin, oncogenes, growth hormones and cell adhesion. It plays a general role in different physiological and pathological processes.\n\nApplications:\nSutiable for use in Antibody Blocking. Other applications not tested.\n\nRecommended Dilutions:\nAntibody Blocking: Use equal volume of S1013-87A with S1013-87. Incubate for 30 minutes at 37 degrees C.\nOptimal dilutions to be determined by researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S1013-87A

Size

50ug

Form

Supplied as a liquid in PBS, pH 7.2, 0.1% BSA, 0.02% sodium azide.

Purity

Purified

References

1. Kharitonenkov, A., et al., Nature 386: 181-186 (1997). 2. Fujioka, Y., et al., Mol. Cell Biol. 16: 6887-6899 (1996). 3. Yamao, T., et al., Biochem. Biophys. Res. Commun. 231: 61-67 (1997). 4. Brooke, G.P., et al., Eur. J. Immunol. 28: 1-11 (1998). 5. Ohnishi, H., et al., J. Biol. Chem. 271: 25569-25574 (1996). 6. Ohnishi, H., et al., Genomics 40: 504-506 (1997). 7. Comu, S., et al., J. Neurosci. 17: 8702-8710 (1997). 8. Stofega, M.R., et al., J. Biol. Chem. 273: 7112-7117 (1998).

Additional Info

Synthetic peptide corresponding to aa487-503, KPEPSFSEYASVQVPRK of human SIRPa1. The sequences of immunogenic peptide differ from those of mouse, rat and bovine by one amino acid.