Tumorigenecity:Yes, in nude mice; forms large cell carcinoma consistent with hepatoma
Isoenzyme:Me-2,2;PGM3,1-2;PGM1,1;ES-D,1-2;AK-1,1;GLO-1,1-2;G6PD,B
Histopathology:adenocarcinoma; ascites
Subculture:Remove medium, rinse with fresh 0.25% trypsin solution, remove trypsin and let the culture sit at room temperature (or at 37�C) until the cells detach (about 10 min). Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days.
Split ratio:1:2 to 1:4
Media change:2 to 3 times weekly
Reverse transcritase: Production
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