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SK-MEL-5

SK-MEL-5

Cat no: CSC-C9606L

Supplier: Creative Bioarray
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Tumorigenecity: Yes, in nude mice; forms malignant melanoma
Isoenzyme: PGM1,1-2;PGM3,1;ES-D,1;AK-1,1;GLO-1,1-2;G6PD,B
Histopathology: malignant melanoma; skin; from metastatic site: axillary node
Subculture: Remove medium, add fresh 0.25% trypsin solution for 1 minute, remove trypsin and let the culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days.
Split ratio: 1:3 to 1:6
Media change: 2 to 3 times per week
Reverse transcritase: Production
Catalogue number: CSC-C9606L
Size: 1.0 mL
Storage temp: Frozen with 52.5% RPMI-1640, 40% FBS, 7.5% DMSO at about 4-5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

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