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Sm/RNP Antigen

Cat no: S1014-45

Sm/RNP Antigen

Heterogeneous nuclear ribonucleoproteins (hnRNP) bind to nascent RNA polymerase II transcripts and are involved in transcript-specific packaging as well as alternative splicing of pre-mRNAs. hnRNPs also appear to influence mRNA metabolism and transport. In vivo, the M proteins bind to mRNA, and in vitro they bind avidly to poly(G) and poly(U) homopolymers. Four different isoforms of hnRNP are produced by alternative splicing: M1, M2, M3, and M4. Ongoing studies have theorized that hnRNPs may cause certain genetic diseases such as myotonic dystrophy, which is the most common form of adult muscular dystrophy. The identification of hnRNPs may therefore be important to determining the cause and cure for genetic diseases.\n\nApplications: \nSuitable for use in ELISA. Other applications have not been tested.\n\nRecommended Dilution:\nELISA: 0.5-1.0ug/ml coating concentration.\n\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S1014-45

Size

200ug

Applications

ELISA

Form

Supplied as a liquid in 20mM Tris-HCI, 0.32M sodium chloride, 10mM magnesium chloride, 0.2mM DTT, pH 7.4, 0.1mM PMSF, 20% glycerol, 0.1% bromo-nitro-dioxane/ methylisothiazoline.

Purity

(same/more than) 90% (SDS-PAGE); Purified by immunoaffinity chromatography.

Read more on Supplier website

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

More info

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

More info
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