Smac (Diablo Homolog, Mitochondrial, Direct IAP-binding Protein with Low pI, Second Mitochondria-derived Activator of Caspasem DIABLO, SMAC)

Recently, three different laboratories have identified a molecule, Smac/DIABLO, which binds with inhibitor of apoptosis proteins (IAPs) and neutralizes IP inhibition of effector caspases. Multiple IAP molecules, including c-IAP1, c-IAP2, XIAP, and survivin are able to bind Smac. Smac/DIABLO is a mitochondrial protein, which is released into cytosol during apoptosis. The function of Smac is similar to that of Drosophila apoptosis activating proteins Reaper, Hid and Grim, which have been proposed to function by neutralizing the activity of IAPs. Smac promotes caspase-3 and caspase-9 activation by removing inhibitors rather than directly acting on Apaf-1, procaspases, and cytochrome c. The full-length cDNA of Smac encodes an open reading frame of 239aa, predicting a protein with a molecular weight of 27kD. However, the mature protein purified from HeLa cells is only about 25kD.\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.5-2ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nHeLa cell lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S1014-48M

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.

P Type

Pab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG

References

1. Du C, et al. Smac, a mitochondrial protein that promotes cytochrome c-dependent caspase activation by eliminating IAP inhibition. Cell 102 (1): 33-42 (2000). 2. Chai J, et al. Structural and biochemical basis of apoptotic activation by Smac/DIABLO. Nature 406 (6798): 855-862 (2000). 3. Srinivasula SM, et al. Molecular determinants of the Caspase-promoting activity of Smac/DIABLO and its role in the death receptor pathway. J. Biol. Chem. Aug 18 [epub ahead of print] (2000).

Additional Info

Recognizes human Smac.