Smac (Second Mitochondria-derived Activator of Caspase, Smac Protein, 0610041G12Rik, DBOH, Direct IAP-binding Protein with Low pI, Diablo, Diablo Homolog (Drosophila), Diablo Homolog, Diablo Homolog Mitochondrial Precursor, DIABLO S, DIABLO-S, FLJ10537, F

Smac/DIABLO, a mitochondrial protein, activates various forms of apoptosis. This activation may be due to the neutralization of one or more members of the IAP family of apoptosis inhibitory proteins. Smac exits the mitochondria and enter the cytosol during certain apoptosis triggered events. Mitochondrial-mediated apoptosis is important in animal development and tissue homeostasis, with alterations resulting in a range of malignant disorders. Upon apoptotic stimuli, the mitochondrial proteins cytochrome c and Smac/DIABLO are released into the cytosol. The release of these proteins, however, occurs via different mechanisms (2,3).\n\nApplications: \nSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:500. Detects a band at ~25kD representing Smac/DIABLO. For better results, IP prior to Western Blot is recommended.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S1014-49L

Size

200ul

Applications

IP, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat

Form

Supplied as ascitic fluid with 0.1% sodium azide

P Type

Mab

Purity

Ascites

Isotype

IgG3+IgG1

References

1. Guo, Y., et al. Caspase-2 Induces Apoptosis by Releasing Proapoptotic Proteins from Mitochondria. JBC Papers in Press. Published on February 6, 2002 as Manuscript M108029200.\n2. Adrain, C., et al. Apoptosis-associated release of Smac/DIABLO from mitochondria requires active caspases and is blocked by Bcl-2. EMBO J. 20(23): 6627-6636, 2001.\n3. Shi, Y. A structural view of mitochondria-mediated apoptosis. Nat. Struct. Biol. 8(5): 394-401, 2001

Additional Info

Recognizes human Smac/DIABLO. Species Crossreactivity: rat and mouse