Smad2 (SMAD-2, hMAD-2, hSMAD2, JV18-1, JV18, MAD-related Protein 2, MADR2, MGC22139, MGC34440, Mothers Against Decapentaplegic Homolog 2, Mothers Against DPP Homolog 2, MADH2)

SMAD2 or Mothers against decapentaplegic homolog 2 is a polypeptide that, as its name describes, is a homolog of the Drosophila gene: "Mothers against decepentaplegic". It belongs to the SMAD family of proteins, which belong to the TGFbeta superfamily of modulators. Like many other TGF beta family members SMAD2 is involved in cell signalling. SMAD2 modulates signals of activin and TGFbeta's. It interacts with SMAD anchor for receptor activation (SARA). The binding of ligands causes the phosphorylation of the SMAD2 protein and the dissociation from SARA and the association with SMAD4. It is subsequently transferred to the nucleus where it forms complexes with other proteins and acts as a transcription factor. SMAD2 is a receptor regulated SMAD (R-SMAD) and is activated by bone morphogenetic protein type 1 receptor kinase.\n\nApplications: \nSuitable for use in Flow Cytometry, Western Blot, Immunohistochemistry, and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: 1:20\nWestern Blot: 1:500-1000\nImmunohistochemistry (paraffin): 1:50\nImmunocytochemistry: 1:250-500\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nJurkat cell lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S1014-52G

Size

100ul

Applications

FC, ICC, IHC, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.01% sodium azide, 0.05% BSA, 40% glycerol.

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1. Eppert, K., et al. (1996). Cell 86: 543-552. 2. Derynck, R., et al. (1996). Curr. Biol. 6: 1226-1229. 3. Yagi, K., et al. (1999). J Biol Chem, Vol. 274, Issue 2, 703-709. 4. Chen, X.,et al. (1996). Nature 383: 691-696. 5. Labb

Additional Info

Recognizes human SMAD2 at and around the MH2 domain.