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Smad4, Recombinant, Human (DPC4, Small Mothers Against Decapentaplegic Deleted in Pancreatic Carcinoma)

Cat no: S1014-54F1

Smad4, Recombinant, Human (DPC4, Small Mothers Against Decapentaplegic Deleted in Pancreatic Carcinoma)

SMAD4 belongs to the SMAD family of proteins that mediate signal transduction by the TGF-beta/activin/BMP-2/4 cytokine superfamily from receptor Ser/Thr protein kinases at the cell surface to the nucleus. SMAD4 promotes the binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides the function of activation required for SMAD1 or SMAD2 to stimulate transcription and may also act as a tumor suppressor. \n\nRecombinant SMAD4 protein was expressed in E. coli and purified by using conventional chromatography techniques.\n\nSource:\nHuman SMAD4 protein, His-tagged was expressed in E. coli.\n\nAA Sequences:\nMGSSHHHHHH SSGLVPRGSH MDNMSITNTP TSNDACLSIV HSLMCHRQGG ESETFAKRAI ESLVKKLKEK KDELDSLITA ITTNGAHPSK CVTIQRTLDG RLQVAGRKGF PHVIYARLWR WPDLHKNELK HVKYCQYAFD LKCDSVCVNP YHYERVVSPG IDLSGLTLQS NAPSSMMVKD EYVHDFEGQP SLSTEGHSIQ TIQHPPSNRA STETYSTPAL LAPSESNATS TANFPNIPVA STSQPASILG GSHSEGLLQI ASGPQPGQQQ NGFTGQPATY HHNSTTTWTG SRTAPYTPNL PHHQNGHLQH HPPMPPHPGH YWPVHNELAF QPPISNHPAP EYWCSIAYFE MDVQVGETFK VPSSCPIVTV DGYVDPSGGD RFCLGQLSNV HRTEAIERAR LHIGKGVQLE CKGEGDVWVR CLSDHAVFVQ SYYLDREAGR APGDAVHKIY PSAYIKVFDL RQCHRQMQQQ AATAQAAAAA QAAAVAGNIP GPGSVGGIAP AISLSAAAGI GVDDLRRLCI LRMSFVKGWG PDYPRQSIKE TPCWIEIHLH RALQLLDEVL HTMPIADPQP LD\n\nStorage and Stability:\nFor long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S1014-54F1

Size

100ug

Form

Supplied as a liquid in 20 mM Tris-HCl buffer, pH 8.0, 20% glycerol.

Purity

(same/more than) 90% (SDS-PAGE)

References

1. Shi Y., et al. (1997) Nature. 388(6637):87-93. 2. de Caestecker MP, et al. (1997) J Biol Chem. 272(21):13690-6.

Alternative Names

SMAD family member 4, DPC4, JIP, MADH4

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