Smad5 phosphorylated (Ser463/465) (Small Mothers Against Decapentaplegic Deleted in Pancreatic Carcinoma)

Smad5 is a member of the Mothers Against Dpp (MAD)-related family of proteins. So far, eight Smads have been identified and can be divided into 3 subgroups based on their structure and function; pathway-restricted, common mediator and inhibitory. Smad 1, 5 and 8 serve as pathway-restricted Smads for the bone morphogenetic proteins (BMPs) signaling pathway (1). Smad5 is directly activated by BMP type Ia or Ib receptors upon BMP-2 stimulation and transduced BMP-2 signals to the nucleus by forming a complex with Smad 4, activating the transcription of the target gene in the nucleus (2-3). BMP activates Smad5 by phosphorylation at Ser463 and Ser465, which are the equivalent phosphorylation sites for Smad 1 and Smad 8.\n\nApplications: \nSuitable for use in Immunocytochemistry, Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:500\nImmunocytochemistry: 1:50 -100\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. \n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

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SPECIFICATIONS

Catalog Number

S1014-55G3

Size

100ul

Applications

ICC, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in 50 mM Tris-Glycine, pH 7.4, 0.15 M sodium chloride, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1. Eppert et al. Cell 86: 543-552, 1996 2. Maci?as-Silva, M., Hoodless, P. A., Tang, S. J., Buchwald, M., and Wrana, J. L. (1998) J. Biol. Chem. 273, 25628-25636 3. Abdollah, S., Maci?as-Silva, M., Tsukazaki, T., Hayashi, H., Attisano, L., and Wrana, J. L. (1997) J. Biol. Chem. 272, 27678-27685

Additional Info

Recognizes human Smad5 phosphorylated at Serine 463 and 465.

Alternative Names

SMAD 5, Mothers against DPP homolog 5, Smad5, hSmad5, JV5-1