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SmiI (SwaI)

Cat no: S1014-59

SmiI (SwaI)

5'-A T T T^A A A T-3' \n3'-T A A A^T T T A-5'\n\nSource:\nStreptococcus milleri S\n\nConcentration:\n10units/ul \n\nUnit Definition:\nOne unit is defined as the amount of SmiI required to digest 1ug of Ad2 DNA-SspI fragments in 1 hour at 30 degrees C in 50ul of assay buffer.\n\nStorage Buffer: \n10mM Tris-HCl (pH 7.4, 25 degrees C) 200mM NaCl, 1mM DTT, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA, 50% glycerol.\n\nSupplied With:\nR1625 Restriction Enzyme Buffer A: 10X: 10mM Tris-HCl (pH 7.4, 25 degrees C) 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA, 50% glycerol. For longer periods, the Storage Buffer should be used.\n\nR1625-03 Restriction Enzyme Buffer D: 10X: 50mM Tris-HCl, pH 7.5, 10mM MgCl2, 100mM sodium chloride, 0.1mg/ml BSA.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S1014-59

Size

200U

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