Smoothelin (FITC) (Smoothelin-A, short smoothelin, smoothelin-b)

Smoothelin is one of the smooth muscle cell (SMC) specific proteins such as calponin, alpha smooth muscle cell actin, SM22alpha and smooth muscle myosin heavy chain isoforms. Smoothelin is exclusively expressed in fully differentiated (contractile) SMCs. It colocalizes with actin stress fibers, this distribution indicates a correlation between smoothelin expression and smooth muscle tissue contractility. Two major isoforms of smoothelin have been reported, a 59kD smoothelin-A in visceral smooth muscle cells and a 110kD smoothelin-B in vascular smooth muscle cells. Both gene products are encoded by a single copy gene which is located on chromosome 22 and regulated by a dual promoter system in a tissue specific manner. The variation in the smoothelin proteins is achieved by alternative splicing in the calponin homology domain. Antibodies to smoothelin are powerful tools in evaluation of smooth muscle cell differentiation as occurs in atherosclerotic lesions.\n\nApplications:\nSuitable for use in Immunofluorescence, Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: 1:50\nWestern Blot: 1:50\nImmunohistochemistry (Frozen): 1:50\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S1014-62H

Size

100ug

Applications

FC, IF, IHC, WB

Hosts

Mouse

Reactivities

Hum, Goat, Rabbit

Form

Supplied as a liquid in PBS, 1% BSA, 0.02% sodium azide. Labeled with fluorescein isothiocyanate (FITC).

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Rensen, S et al; Expression of the smoothelin gene is mediated by alternative promoters. Cardiovasc Res 2002, 55: 850 2. Saleem, M et al; The molecular and functional phenotype of glomerular podocytes reveals key features of contractile smooth muscle cells. Am J Physiol Renal Physiol 2008, 295: F959

Additional Info

Recognizes human Smoothelin. Reacts with both smoothelin-A and smoothelin-B. Does not crossreact with canine, porcine or mouse. Species Crossreactivity: goat, rabbit

Alternative Names

Smoothelin-A, short smoothelin, smoothelin-b