Smoothelin

The antibody discriminates fully differentiated contractile smooth muscle cells (SMC) from proliferative SMC. Myofibroblasts, myoepithlial cells, skeletal and cardiac muscle do not contain smoothelin. The distribution of smoothelin-positive cells indicates a correlation between smoothelin expression and smooth muscle tissue contractility. The antibody is very useful for characterization of atherosclerotic lesions. \n\nApplications:\nSuitable for use in Western Blot, Immunofluorescence microscopy and Immunohistochemistry. Other applications have not been testd.\n\nRecommended Dilution:\nWestern Blot: 1:1000 using ECL\nImmunohistochemistry (frozen and paraffin embedded): 1:100-1:200 with PBS, pH 7.4. Microwave pretreatment required. Use ABC method. Incubate 1hour at RT; extend time for paraffin embedded tissue.\nOptimal dilution determined by the researcher. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S1014-62B

Size

100ul

Applications

IF, IHC, WB

Hosts

Mouse

Reactivities

Hum, Can, Ch/Bird, Fel, NHP, Prc

Form

Supplied as a liquid in 0.09% sodium azide.

P Type

Mab

Purity

Hybridoma culture supernatant.

Isotype

IgG1

References

1. Van der Loop FT, et al., Smoothelin, a novel cytoskeletal protein specific for smooth muscle cells. J Cell Biol 134, 401-411 (1996). 2. Wehrens XHT, et al., Localization of smoothelin in avian smooth muscle and identification of a vascular-specific isoform. FEBS Letters 405, 315-320 (1997). 3. Van der Loop FTL, et al., Differentiation of smooth muscle cells in human blood vessels as defined by smoothelin, a novel marker for the contractile phenotype. Arterioscler Thromb Vasc Biol 17, 665-671 (1997)

Additional Info

Recognizes chicken Smoothelin. A 59kD isoform of smoothelin is present in visceral SMC and a 100kD isoform is present in vascular SMC. Species Crossreactivity: Monkey, canine, feline, porcine, human.