SMUG1 (Single-strand Selective Monofunctional Uracil DNA Glycosylase)

Cat no: S1014-65D3

Supplier: United States Biological

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SMUG1 is a glycosylase that removes uracil from single- and double-stranded DNA in nuclear chromatin, thus contributing to base excision repair. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:32,000 Western Blot: 0.5-2ug/ml, observed in Molt-4 lysates on ~35kD bands Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Catalogue number:	S1014-65D3
Reactivities:	Human
Hosts:	Goat
Applications:	ELISA, Western Blot
Size:	100ug
Form:	Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
P type:	Pab
Purity:	Purified by immunoaffinity chromatography.
References:	1. Ma KW, Au SW, Waye MM. Over-expression of SUMO-1 induces the up-regulation of heterogeneous nuclear ribonucleoprotein A2/B1 isoform B1 (hnRNP A2/B1 isoform B1) and uracil DNA glycosylase (UDG) in hepG2 cells. Cell Biochem Funct. 2009 Jun;27(4):228-37.
Additional info:	Recognizes human SMUG1.

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SMUG1 (Single-strand Selective Monofunctional Uracil DNA Glycosylase)

Cat no: S1014-65D3

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