SNAP 25, Recombinant, C. elegans (Synaptosomal-Associated Protein 25)

SNAP-25 is a presynaptic plasma membrane protein essential for synaptic vesicle fusion and neurotransmitter release . The membrane proteins SNAP-25, synaptobrevin and syntaxin make up the core complex (SNARE) essential for docking and holding synaptic vesicles at the pre synaptic membrane.\n\nDescription: \nSNAP-25 gene was amplified by PCR from C.elegans and cloned into an E. coli expression vector. SNAP-25 was purified by using conventional chromatography techniques. Recombinant C.elegans SNAP-25 produced in E.Coli is a single,non-glycosylated polypeptide chain containing 207 amino acids and having a molecular mass of 23kD.\n\nSequence:\nMSGDDDIPEG LEAINLKMNA TTDDSLESTR RMLALCEESK EAGIKTLVML DDQGEQLERCEGALDTINQD MKEAEDHLKG MEKCCGLCVL PWNKTDDFEK TEFAKAWKKD DDGGVISDQPRITVGDSSMG PQGGYITKIT NDAREDEMDE NVQQVSTMVG NLRNMAIDMS TEVSNQNRQL DRIHDKAQSN EVRVESANKR AKNLITK\n\nStability: \nStore at 4 degrees C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degrees C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

S1014-71C

Size

10ug

Form

Supplied as a liquid in 20mM Tris-HCl pH 7.5, 50mM sodium chloride, 5mMDTT, 1mM EDTA and 10% glycerol.

Purity

(same/more than) 95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.

References

1. The SNAP-25 gene is associated with cognitive ability: evidence from a family-based study in two independent Dutch cohorts.\n\nMol Psychiatry 2006 Jun 27;\n\n2. Antibody inhibition of synaptosomal protein of 25 kDa (SNAP-25) and syntaxin 1 reduces rapid exocytosis in insulin-secreting cells.\n\nJ Mol Endocrinol 2006 Jun;36(3):503-15\n\n3. CytLEK1 Is a Regulator of Plasma Membrane Recycling through Its Interaction with SNAP-25.\n\nMol Biol Cell 2006 Jul;17(7):3176-86\n\n4. Mouse taste cells with G protein-coupled taste receptors lack voltage-gated calcium channels and SNAP-25.\n\nBMC Biol 2006 Mar 30;4:7\n\n5. Increased levels of SNAP-25 and synaptophysin in the dorsolateral prefrontal cortex in bipolar I disorder.\n\nBipolar Disord 2006 Apr;8(2):133-43\n\n6. Modulation of Kv2.1 channel gating and TEA sensitivity by distinct domains of SNAP-25.\n\nBiochem J 2006 Jun 1;396(2):363-9