SNAP 25 (SNAP25, SP-12, Synaptosomal-Associated protein 25, SNAP-25, Synaptosomal-Associated 25kD Protein, SUP, Super Protein, SNAP-25B)

Synaptosomal-associated protein 25 (SNAP-25) is a membrane bound protein anchored to the cytosolic face of membrane via palmitoyl side chains in the middle of the molecule (1). It is a component of the SNARE complex, which is proposed to account for the specificity of membrane fusion and to directly execute fusion by forming a tight complex that brings the synaptic vesicle and plasma membranes together (2). SNAP25 exists as two alternatively spliced isoforms, SMAP25A and SNAP25B. These isoforms are differentially expressed in neurons and neuroendocrine cells and they differ by 9aa in the central portion of the protein (3).\n\nApplications: \nSuitable for use in Immunofluorescence, ELISA, Western Blot and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunofluorescence: 1:1000\nELISA: 1:5000\nWestern Blot: 1:1000\nImmunohistochemistry: 1:1000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

136848

Size

25ug

Applications

ELISA, ICC, IF, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Fel, Prc

Form

Supplied as a liquid in PBS, pH 7.4, 50% glycerol.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Hodel A. (1998) Int J. Biochem Cell Biol. 30(10): 1069-1073. 2. Sudhof T.C., and Rizo J. (2002) Nat Rev Neurosci. 3(8): 641-653. 3. Puffer E.B., et al. (2001) Biochemistry. 40(31): 9374-9378.

Additional Info

Recognizes human SNAP-25 at ~29kD. Species Crossreactivity: Mouse, rat, feline, and porcine.

Alternative Names

SNAP25, SP-12, Synaptosomal-Associated protein 25, SNAP-25, Synaptosomal-Associated 25kD Protein, SUP, Super Protein, SNAP-25B