SNAP 25 (Synaptosomal-associated Protein 25, SNAP-25, bA416N4.2, dJ1068F16.2, FLJ23079, HGNC:11132, Resistance to Inhibitors of Cholinesterase 4 Homolog, RIC4, RIC-4, SEC9, SNP25, Super Protein, SUP)

Research studies have used this antibody to study the distribution of synaptic changes in the hippocampus of patients with medically refractory temporal lobe epilepsy (1, 3, 8). \n\nApplications:\nSuitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested. \n\nRecommended Dilution: \nImmunohistochemistry (Paraffin): 1:2000-1:5000\nDoes not require protein digestion or antigen retrieval using heat treatment prior to staining of paraffin sections. \nWestern Blot: 1:500-1:5000\nOptimal working dilutions to be determined by researcher.\n\nIHC Positive Control:\nBrain\n\nHybridoma:\nNSO myeloma cells with spleen cells from Balb/c mice. \n\nRecommended Secondary Antibodies:\nI1904-04D: IgG, H&L (AP) Pab Gt xMo\nI1904-06F: IgG, H&L (FITC) Pab Gt xMo\nI1904-06C: IgG, H&L (HRP) Pab Gt xMo\nI1903-07E: IgG, (HRP) Pab Rb xMo\nI1903-13A: IgG, (PE) Pab Gt xMo\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S1014-70F

Size

100ul

Applications

ELISA, IHC, WB

Hosts

Mouse

Reactivities

Hum, Rat, Prc

Form

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG1

References

1. Honer, W.G., et al., 1994, Hippocampal synaptic pathology in patients with temporal lobe epilepsy. Acta Neuropathol. 87: 202-210. 2. Honer, W.G., et al., 1992, Regional synaptic pathology in Alzheimer's disease. Neurobiol. Aging. 13: 375-382. 3. Honer, W.G., et al., 1993, Human synaptic proteins with a heterogeneous distribution in cerebellum and visual cortex. Brain Res. 609: 9-20. 4. Honer, W.G., et al., 1992, Characterization of a synaptic antigen of interest in neuropsychiatric illness. Biol. Phychiatry 31: 147-158. 5. Honer, W.G., et al., 1989, Monoclonal antibodies to study the brain in schizophrenia. Brain Res. 500: 379-383. 6. Masliah, E., et al., 1994, Topographical distribution of synaptic-associated proteins in the neuritic plaques of Alzheimer's disease hippocampus. Acta Neuropathol. 87: 135-142. 7. Simpson, I.A., et al., 1994, Decreased concentrations of GLUT1 and GLUT3 glucose transporters in the brains of patients with Alzheimer's disease. Ann Neurol. 35: 546-551. 8. Wakabayashi, K., et al., 1994, Synapse alterations in the hippocampalentorhinal formation in Alzheimer's disease with and without Lewy body disease. Brain Research 667: 24-32. 9. Dickson, D.W., et al., 1994, Hippocampal sclerosis: a common pathological feature of dementia in very old (>80 years of age) humans. Acta Neuropathologica 88: 212-221. 10. Dickson, D.W., et al., 1995, Correlations of synaptic markers with cognitive status in prospectively studied elderly humans. Neurobiol Aging (in press).

Additional Info

Recognizes human SNAP-25; an antigen of 26-27kD molecular weight. Will react with SNAP-25 from COS cells, but not with the fusion protein from bacterial systems. Species Crossreactivity: rat, hamster and porcine