SNAP 25 (Synaptosomal Associated Protein)

SNAP 25 (synaptosome-associated protein of 25kD) is a SNAP (soluble N-ethylmaleimide-sensitive fusion (NSF) attachment protein) receptor (SNARE-related protein) which is tightly associated with the plasma membrane by means of the palmitoylation of a cluster cystein residues with its structure. SNAP 25 is specifically cleaved close to the carboxyl terminal by the botulinum neurotoxins (BoNT) A and E which are known to inhibit neurotransmitter release. Similar inhibitory effects of BoNT/A and E have been recorded for non-neuronal secretory cells, such as adrenal medulla, This leads to the identification of SNAP 25 in SNARE-like complexes precipitated from chromaffin cells.\n\nApplications:\nSuitable for use in Western Blot, Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000-1:2000 (using ECL). Reacts strongly with a single major band (~25kD) on denaturing Western Blots of bovine adrenal medulla and rat brain fractions. Will not detect SNAP-25 following proteolysis with botulinum neurotoxins A and E.\nOptimal dilutions to be determined by researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S1014-70C

Size

50ul

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Rat, Bov

Form

Supplied as a liquid, 0.1% sodium azide.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Nature 365: 160-163 (1993). 2. Biochem. Biophys. Res. Comm. 212: 945-952 (1995). 3. J. Cell Biol. 109: 3039-3052 (1989). 4. FEBS Lett. 351: 207-210 (1994). 5. Cell 75: 409-418 (1993).

Additional Info

Recognizes SNAP 25 (Synaptosomal-Associated Protein). Will not detect SNAP 25 following proteolysis with botulinum neurotoxins A and E. No crossreactivity expected with SNAP 23. Species Crosseactivity: Bovine and rat.