SNAP23 (Vesicle-membrane fusion protein SNAP-23)

SNAP23(Synaptosomal-Associated Protein, 23-KD), also called SNAP23A, is a protein that in humans \nis encoded by the SNAP23 gene. The SNAP23 gene has 8 exons, with the initiation codon located in \nexon 2. The SNAP23 gene is mapped on 15q15.1-q15.2. The SNAP23 cDNA encodes a 211-amino \nacid polypeptide with a predicted mass of 23 kD. Its amino acid sequence is 59% identical to that of \nSNAP25. Northern blot analysis revealed that SNAP23 is ubiquitously expressed. SNAP23 is able to \nbind to multiple syntaxins as well as to multiple vesicle-associated membrane proteins. After \nrelocation, SNAP23 is required for exocytosis, implying a crucial role in promoting membrane fusion. \nTIVAMP-containing vesicles were concentrated in the apical domain of epithelial cells. STX3A \nand SNAP23 were codistributed at the apical plasma membrane, where they formed N-ethyl \nmaleimide-dependent SNARE complexes with TIVAMP and cellubrevin. SNAP23 is structurally and \nfunctionally similar to SNAP25 and binds tightly to multiple syntaxins and synaptobrevins/VAMPs. It is \nan essential component of the high affinity receptor for the general membrane fusion machinery and is \nan important regulator of transport vesicle docking and fusion.\n\nUniProt Number:\nO00161\n\nGene ID:\nSNAP23\n\nApplications:\nSuitable for use in Western Blot, Immunohistochemistry (Paraffin and Frozen).\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Handling:\nStore at -20?C for one year. After reconstitution, store at 4?C for one month. Can also be aliquoted and stored frozen at -20?C for long term.\nAvoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

145201

Size

100ug

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a lyophilized powder. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Guo, Z., Turner, C., Castle, D. Relocation of the t-SNARE SNAP-23 from lamellipodia-like cell \nsurface projections regulates compound exocytosis in mast cells. Cell 94: 537-548, 1998.\n2. Lazo, P. A., Nadal, M., Ferrer, M., Area, E., Hernandez-Torres, J., Nabokina, S. M., Mollinedo, F., \nEstivill, X. Genomic organization, chromosomal localization, alternative splicing, and isoforms of the \nhuman synaptosome-associated protein-23 gene implicated in vesicle-membrane fusion \nprocesses. Hum. Genet. 108: 211-215, 2001.\n3. Mollinedo, F., Lazo, P. A. Identification of two isoforms of the vesicle-membrane fusion protein \nSNAP-23 in human neutrophils and HL-60 cells. Biochem. Biophys. Res. Commun. 231: 808-812, \n1997.

Additional Info

Recognizes human, mouse and rat SNAP23. No crossreactivity with other proteins.