SNCB, Recombinant, Human (Beta-synuclein)

Synuclein is a small, soluble protein primarily expressed in neural tissue. The synuclein family includes three known proteins: alpha-synuclein, beta-synuclein, and gamma-synuclein. alpha-Synuclein is a very conserved, small acidic protein (140 residues) with a molecular weight of approximately 19kD that accounts for about 1% of total protein in neurons. It is concentrated primarily in presynaptic axon terminals and plays a central role in initiating Parkinson's disease. It is the principal constituent of Lewy bodies which are formed by protein aggregation and inclusion body formation. alpha-Synuclein lacks secondary or tertiary structure, so it belongs to the family of natively unfolded proteins, many of which act as chaperones. Much of this alpha-synuclein has been post-translationally modified to increase the rate of oligomerization. Mutant forms of alpha-Synuclein are associated with autosomal dominant Parkinson's disease. The mutations increase the aggregation rate of the resultant alpha-synuclein beta-Synuclein is found primarily in brain tissue and is seen mainly in presynaptic terminals. It is predominantly expressed in the neocortex, hippocampus, striatum, thalamus, and cerebellum. beta-Synuclein may protect the central nervous system from the neurotoxic effects of alpha-synuclein by inhibiting aggregation of alpha-synuclein which occurs in neurodegenerative diseases such as Parkinson's disease. gamma-Synuclein is a synuclein protein found primarily in the peripheral nervous system (in primary sensory neurons, sympathetic neurons, and motor neurons) and retina. gamma-Synuclein expression in breast tumors is a marker for tumor progression.\n\nSource:\nRecombinant corresponding to aa1-134 with N-His tagm from human beta-synuclein, expressed in E.coli.\n\nMolecular Weight:\n~16.6kD\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S9501-02P

Size

200ug

Form

Supplied as a lyophilized powder from PBS, pH7.4. Reconstitute with sterile dH2O PBS.

Purity

~95% (SDS-PAGE)

References

1. Irvine GB et al. 2008, Mol Med. In press. 2. Wakabayashi K et al. 2007, Neuropathology. 27(5):494-506. 3. Beyer K. Acta Neuropathol. 112(3):237-251. 4. Bruening W et al. 2000, Cancer. 88(9):2154-2163.