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SNIP1, Recombinant, Human, aa258-396, His-tag (Smad nuclear interacting protein 1)

Cat no: S1014-96D

SNIP1, Recombinant, Human, aa258-396, His-tag (Smad nuclear interacting protein 1)

SNIP1 is smad nuclear interacting protein contains a forkhead-associated (FHA) domain and acts as a nuclear inhibitor of CBP/p300. This protein is an inhibitor of the TGF-beta signal transduction pathway and to be important in suppressing transcriptional activation dependent on the co-activators CBP and p300. Inhibition of NF-kappa B activity is a function of the N-terminal domain of SNIP1 and involves competition of SNIP1 and the NF-kappa B subunit, RelA/p65, for binding to p300, similar to the mechanism of inhibition of Smad signaling by SNIP1. Purified by using conventional chromatography techniques.\n\nSource:\nRecombinant human corresponding to aa258-396 of SNIP1, fused to His-tag at N-terminus, expressed in E.coli.\n\nAA Sequence:\nMGSSHHHHHH SSGLVPRGSH MRWRLYPFKN DEVLPVMYIH RQSAYLLGRH RRIADIPIDH PSCSKQHAVF QYRLVEYTRA DGTVGRRVKP YIIDLGSGNG TFLNNKRIEP QRYYELKEKD VLKFGFSSRE YVLLHESSDT SEIDRKDDED EEEEEEVSDS\n\nMolecular Weight: \n~18.8kD (160aa) confirmed by MALDI-TOF\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S1014-96D

Size

100ug

Form

Supplied as a liquid in 20mM Tris-HCl buffer, pH 8, 2mM DTT, 100mM sodium chloride, 20% glycerol.

Purity

~90% (SDS-PAGE)

References

Kim RH, et al. (2000) Genes Dev. 14(13):1605-16. Kim RH, et al. (2001) J Biol Chem. 276(49):46297-304.

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