SOCS, Antibody Sampler Set, BioAssay(TM)

The JAK/STAT cytokine signal pathway is controlled at multiple levels by a number of molecules and different means including a family of proteins termed suppressors of cytokine signalling (SOCS). SOCS family members are emerging as one of the most important feedback inhibitors of cytokine receptor signaling. The mammalian SOCS protein family consists of at least eight distinct members, SOCS1-7 and cytokine-inducible SH2-containing protein (CIS), that share a common structure including a N-terminal region highly variable in length and primary sequence, a central Src Homology 2 (SH2) domain, and a C-terminal SOCS box. Functional analyses in genetically modified mice, whose SOCS proteins are over-expressed or deleted, have demonstrated that the SOCS family members have indispensable roles in immune responses Also, there is increasing evidence that SOCS family was involved in the pathogenesis of many diseases, such as hepatitis-induced carcinogenesis, myeloid leukaemia and the metabolic syndrome. Further research on SOCS proteins may lead to the development of strategies that utilize these proteins for diagnostic and therapeutic purposes. Rabbit anti-SOCS antibodies were raised against synthetic peptides corresponding to the human SOCS-1, SOCS-2, SOCS-3, SOCS-4, SOCS-5 and CIS-1 proteins. Specificity: Species reactivity includes human and mouse. Kit Components: SOCS-1 (Rb x Hu): 1x25ug SOCS-2, CT (Rb x Hu): 1x25ug SOCS-3 (Rb x Hu): 1x25ug SOCS-4 (Rb x Hu): 1x25ug SOCS-5 (Rb x Hu): 1x25ug CIS-1 (Rb x Hu): 1x25ug Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.5-2ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.