SOCS7, ID (Suppressor of Cytokine Signaling 7, SOCS-7, Nck Ash and Phospholipase C gamma-binding Protein, Nck-associated Protein 4, NAP4, NAP-4, NCKAP4)

Suppressors of cytokine signaling (SOCS) protein family includes eight members in humans: SOCS-1 through SOCS-7 and cytokine-inducible Src- homology 2 (SH2) containing protein (CIS). All members of the family have similar structure: variable length (50-380aa) and aa sequence N-terminal region, SH2 domain in the center, and homologous C-terminal 40aa long sequence that was named SOCS box. Studies have demonstrated that animals lacking normal SOCS function exhibit high cytokine activity that leads to a variety of inflammatory and autoimmune diseases and even cancer. SOCS-7 is one of the least studied members of the SOCS family. It is 581aa long and has 56% sequence identity with SOCS-6 in SH 2 region. SOCS-7 is the only member of the SOCS family that contains nuclear localization signal and proline-rich regions; specifically, it can interact with adaptor molecules Nck and Ash. It is predominantly expressed in the testis and brain. Similar to SOCS-6, it binds to IRS-2 and IRS-4 proteins. In addition, SOCS-7 interacts with actin, vinexin, and STAT5.\n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:200-1:500\nELISA: 1:5000-1:20,000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S1040-07E

Size

50ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Hilton D. J. et al. (1998) Biochemistry 195: 114-119. 2. Krebs D. et al. (2001) Stem Cells 19: 378-387. 3. Bullock A. N. et al. (2007) Structure 15 (11): 1493-1504. 4. Nicholson S.E. et al. (2005) PNAS 102 (7): 2328-2333. 5. Kario E. et al. (2005) J. Biol. Chem. 280 (5): 7038-7048. 6. Krebs D.L> et al. (2002) Mol. Cell Biol. 22(13): 4567-4578. 7. Martens N. et al. (2005). J. Biol. Chem. 280(14): 13817-13823.

Additional Info

Recognizes human SOCS-7. Species Crossreactivity: mouse.