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Solute Carrier Family 12 (Potassium-Chloride Transporter) Member 7 (SLC12A7) ELISA Kit

Solute Carrier Family 12 (Potassium-Chloride Transporter) Member 7 (SLC12A7) ELISA Kit

Cat no: ABIN414781

Supplier: Antibodies-online
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Solute Carrier Family 12 (Potassium-Chloride Transporter) Member 7 (SLC12A7) ELISA Kit: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Potassium Chloride Cotransporters 4 (KCC4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Potassium Chloride Cotransporters 4 (KCC4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Potassium Chloride Cotransporters 4 (KCC4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm
Catalogue number: ABIN414781
Size: 96 tests
Accession: Q9Y666
Gene: 10723
Antigen: KCC4
Sensitivity: The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.
Assay range: 0.156-10 ng/mL
Sample type: Tissue Homogenate,Biological Fluids
Entrez gene: 10723

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