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solute carrier family 9a3 (Slc9a3, (sodium/hydrogen exchanger), member 3, Na(+)/H(+) exchanger 3, Nhe3, NHE-3, Sodium/hydrogen exchanger 3)

Cat no: S5329-10G

solute carrier family 9a3 (Slc9a3, (sodium/hydrogen exchanger), member 3, Na(+)/H(+) exchanger 3, Nhe3, NHE-3, Sodium/hydrogen exchanger 3)

Sodium-hydrogen exchanger 3 (NHE3; Slc9a3) is an epithelial transport protein that carries out 1:1 exchange of Na+ and H+ across the plasma membrane. It is apically located in the prroximal tubule of the kidney, the thick ascending limb of the kidney, and in small intestine (1). NHE3 is phosphorylated and regulated by multiple kinases including PKA, SGK1 and CK2. It can be phosphorylated by calyculin A, and dephosphorylated by PP1 catalytic subunit in vitro (2).\n\nApplications: \nSuitable for use in Western Blot and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S5329-10G

Size

100ul

Applications

ICC, WB

Hosts

Rabbit

Reactivities

Mouse, Rat

Form

Supplied as a liquid, 50% glycerol.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Kim G.H., Ecelbarger C.A., Knepper M.A. and Packer R.K. (1999) J Am Soc Nephrol. 10: 935-942.\n2. Dynia D.W., Steinmetz A.G. and Kocinsky H.S. (2010) Am J Physoil Renal Physiol. 298(3): F745-F753.

Additional Info

Recognizes rat Slc9a3. Species Crossreactivity: Mouse

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