Somatostatin

Recognizes the cyclic polypeptide hormone identified as Somatostatin. Somatostatin was originally isolated from the hypothalamus and characterized by its ability to inhibit release of growth hormone from the pituitary gland. It exists in two forms, somatostatin-14, composed of 14 amino acids and somatostatin-28, a prohormone composed of 28 residues. The Somatostatin antibody will label D cells of the endocrine mammalian pancreas and also cells of the hypothalamic parvicellular region. It may prove useful for the identification of tumors and hyperplasias of the pancreatic islets.\n\nApplications:\nSuitable for use in Immunohistochemistry (formalin/paraffin). Other applications not tested.\n\nRecommended Dilution: \nImmunohistochemistry: 1:50-1:100 for 30 min at RT.\nNote: Staining of formalin fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0.\n\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nPancreas\n\nCellular Localization: \nCytoplasmic\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C or colder. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S5331-11

Size

500ul

Applications

IHC

Hosts

Rabbit

Reactivities

Bov

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide, 0.2% BSA.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Alumets J, et al. Distribution, ontogeny and ultrastructure of somatostatin immunoreactive cells in the pancreas and gut. Cell Tiss Res 1977; 185:465. 2. Walsh J H. Gastrointestinal hormones, in Johnson L R (ed). Physiology of the Gastrointestinal Tract, 2nd ed, New York, Raven Press, 1810253 (1987). 3. Keast J R, Furness J B and Costa M. Somatostatin in human enteric nerve. Distribution and characterization. Cell Tissue Res. 237: 299-308 (1984).

Additional Info

Human Somatostatin. Species crossreactivity: Others not known.