Sonic Hedgehog (C-Product) (Sonic Hedgehog Homolog (Drosophila), HHG1, HHG-1, HLP3, HPE3, MCOPCB5, SMMCI, Sonic Hedgehog Protein, TPT, TPTPS)

Sonic Hedgehog Protein (SHH, VHH-1) belongs to hedgehog protein family which includes Indian Hh, and Desert Hh. Hh family is involved in the cell fate and patterning during embryonic development, homeostasis, and adult tissue renewal. Similar to other member in the family, SHH binds to the patched (PTC) cell surface receptor, releasing the signal transducer Smoothened (Smo) to transmit the Hh signal into the cell and activate transcription of the target gene. Precursor SHH is autocatlytically cleaved into two subunits, N-terminal and C-terminal products. Soluble N-terminal product is involved in the signaling activity, while C-product displays an autoproteolysis activity on the precursor and a cholesterol transferase activity on the N-terminal product. C-terminal product attaches a cholesterol moiety to the N-terminal product, preventing N-terminal product diffusion within the developing embryo. A defect in SHH has been linked in holoprosencephaly type 3 (HPE3), in which developing forebrain fails to separate into right and left hemispheres, and ocular coloboma.\n\nApplications:\nSuitable for use in Flow Cytometry, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: 1:40\nWestern Blot: 1:2000\nImmunohistochemistry (paraffin): 1:100\nImmunoprecipitation: 1:70\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nFetal liver membrane, human kidney tissue.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S1012-96L

Size

100ul

Applications

FC, IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in 50mM Tris-Glycine, pH7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide, 0.05% BSA.

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1. Taipale, J., et al. (2001). Nature. 411:349

Additional Info

Recognizes both full length (50kD) and c-product subunit (27kD) of human SHH protein. Sonic hedgehog protein C-product is secreted and can be found in the extracellular space.