Sox 15 (High Mobility Group (HMG) Transcription Factor, SRY (Sex Determining Region Y)-Box 15, SRY-Box 20)

SOX15 (also known as SOX20) is a nuclear, 25 kD transcriptional repressor that belongs to group G of the SOX gene family. Human SOX15 is 233 amino acids (aa) in length and contains a 63 aa DNA-binding HMG-box (amino acids # 48-110). Although the C-terminus (aa # 126-233) does not bind DNA, it is required for SOX15 repressive activity. It may accomplish this by interacting with b-catenin. SOX15 is expressed in myoblasts and regulates skeletal muscle regeneration and differentiation. Human SOX15 shares 75% aa sequence identity with mouse SOX15.\n\nApplications: \nWestern Blot: This antibody can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect human SOX15. The detection limit for rhSOX15 is approximately 20 ng/lane and 5 ng/lane under non-reducing and reducing conditions, respectively. In this format, this antibody shows less than 1% cross-reactivity with rhSOX1, -2, -5, -6, -7, -10, -12, -14, -15, -17, and rhSOX21. \nImmunocytochemistry: This antibody can be used at a concentration of 10ug/ml to detect SOX15 in NTera2 teratocarcinoma cells. Cells were fixed with PBS containing 4% paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4 degrees C followed by Rhodamine RedTM-coupled anti-sheep IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing 0.1% BSA. \nDirect ELISA: This antibody can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect human SOX15. The detection limit for rhSOX15 is approximately 0.3 ng/well. \nOptimal dilutions should be determined by each laboratory for each application.\n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S5364-44C

Size

100ug

Applications

ELISA, ICC, WB

Hosts

Sheep

Reactivities

Hum

Form

Supplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS, 40-50% glycerol. If 0.5ml is used, the concentration will be 0.2mg/ml.

P Type

Pab

Purity

Purified by human SOX15 affinity chromatography.

Isotype

IgG

Additional Info

Selected for its ability to recognize human SOX15 in the applications listed.