Sox 2, Mouse, Rat, Primer Pair (SOX2, SOX-2, Sex Determining Region Y-box 2, SRY (Sex Determining Region Y) Box 2, SRY-related HMG Box 2, ANOP3, MCOPS3, MGC2413, Transcription Factor SOX2)

Mouse/rat SOX2 (SRY-box containing gene 2) specific PCR? primers for RT-PCR (reverse transcription followed by polymerase chain reaction) analysis of mRNA expression. \n\nComponents:\nPrimer Pair: Lyophilized. Each vial contains 375 pmoles of each primer. Adjust to a final concentration of 7.5 pmoles/ul by resuspending Primer Pair in either 50ul autoclaved deionized water or 0.1X TE buffer (1mM Tris HCl, pH8.0 @ 25 degrees C; 0.1mM EDTA, pH 8.0 @ 25 degrees C). \n\nPositive Control 59: Lyophilized. Each vial contains 150 ng synthetic double-stranded DNA. Resuspend in 30ul autoclaved deionized water or TE buffer. To prevent contaminating samples and reagents, it is strongly recommended that the Positive Control is resuspended in a separate location from where PCR reactions are set up. Use a different pipette than one used for PCR set up. The Positive Control is not intended for quantitative purposes.\n\nGenBank Accession Number: \nNM_011443 (mouse) and XM_574919 (rat) \n\nProduct Sizes: \ncDNA: 406 base pairs (bp) (mouse), 423 bp (rat) \nGenomic DNA: 406 bp product predicted and observed for mouse. Sequence not available for rat. Product observed at 423 bp for rat. Treatment of RNA with RNase-free DNase is recommended to remove contaminating genomic DNA. \nPositive Control 59: 290 bp \nAlternate splice variants: None reported. \n\nSuggested Reaction Conditions: \nFor each 50ul Polymerase Chain Reaction mixture, 2ul of the primer pair stock solution should be used. Sufficient reagent is supplied for 25 separate amplification reactions of the gene fragment.\n\nThermocycling Conditions: \nA typical 50ul PCR would involve a 4 minute denaturation step at 94C, followed by 30-35 cycles of 45 seconds at 94C, 45 seconds at 55C and 45 seconds at 72C, with a final 10 minute extension step at 72C.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sutoclaved ddH2O or 0.1X TE buffer. Aliquot and store at -20 degrees C. Stable for only one thaw. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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