Sox 2 (SOX2, SOX-2, Sex Determining Region Y-box 2, SRY (Sex Determining Region Y) Box 2, SRY-related HMG Box 2, ANOP3, MCOPS3, MGC2413, Transcription Factor SOX2)

Embryonic stem cells are derived from the inner cell mass of the blastocyst and are unique in their pluripotent capacity and potential for self-renewal. Sox2 is one of a set of transcription factors that are crucial for the maintenance of pluripotency (1). Sox2, Oct-4 and Nanog cooperate in this network (1-3) and siRNA knockdown of either Sox2 or Oct-4 results in loss of pluripotency (4,5). Chromatin immunoprecipitation experiments have shown that Sox2 and Oct-4 bind to thousands of gene regulatory sites, highlighting the importance of these transcription factors in early embryonic development (6,7). It has recently been shown that Sox2 is amplified in lung and esophageal squamous cell tumors (8).\n\nApplications:\nSuitable for use in Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nChromatin Immunoprecipitation (CHIP): 1:25.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S5364-11H

Size

200ul

Applications

IP

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in 10 mM HEPES pH 7.5, 150 mM NaCl, 0.1mg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1. Nichols, J. et al. (1998) Cell 95, 379-391, 2. Avilion, A.A. et al. (2003) Genes Dev. 17, 126-140, 3. Rodda, D.J. et al. (2005) J. Biol. Chem. 280, 24731-24737, 4. Matin, M.M. et al. (2004) Stem Cells 22, 659-668, 5. Niwa, H. et al. (2000) Nat. Genet. 24, 372-376, 6. Boyer, L.A. et al. (2005) Cell 122, 947-956, 7. Loh, Y.H. et al. (2006) Nat. Genet. 38, 431-440.

Additional Info

Recognizes endogenous levels of human total Sox2 protein. Species sequence homology: monkey, bovine, canine and equine.