Sox 9 (High Mobility Group (HMG) Transcription Factor)

SOX9 is a member of the family of SOX (Sry-type high mobility group box) gene that were first identified on the basis of region with high homology to that of Sry (Sex-determining region Y) (1). It is a transcription factor with a high mobility group DNA-binding domain that is expressed in all prechondrocytic and chondrocytic cells during embryonic development in a pattern that closely parallels that of the gene for type II collagen (2). Phosphorylation of SOX9 by PKA increases its DNA binding and transcriptional activity (3). SOX9 regulates expression of chondrocyte phenotype related genes (4,5). IL-1 and TNF-a can cause a marked and rapid decrease in the levels of SOX9 mRNA and/or protein in chondrocytes, thus strongly inhibiting the expression of genes for cartilage extracellular matrix proteins (6). The down-regulation of Sox9 may have a crucial role in inhibiting expression of the cartilage phenotype in inflammatory joint diseases.\n\nApplications: \nSuitable for use in ELISA, Dot Blot, Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nELISA: 0.1-1ug/ml\nWestern Blot: 0.5-2ug/ml; Detects Sox9 in mouse embryo cell lysate (Band at ~65kD). Samples were boiled prior to 10% SDS-PAGE.\nImmunoprecipitation: 3-5ug/extract from 10e6 cells.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S5364-26

Size

50ug

Applications

ELISA, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Bov

Form

Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.05% sodium azide, before the addition of glycerol to 40%.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

US Biological application reference: de Bont, J.M. et al. (2008) Neuro-Oncology 10:648-660. 1. Wright, E.M., et al., Nucleic Acids Res. 21: 744 (1993). 2. Sekiya, I., et al., J. Biol. Chem. 275(15): 10,738 (2000). 3. Crombrugghe, B., et al., Matrix Biol. 19(5): 389 (2000). 4. McDowall, S., et al., J. Biol. Chem. 274(34): 24,023-24,030 (1999). 5. Koopman, S.I., et al., J. Endocrinol. 169(3): 573-579 (2001). 6. Murakami, S., et al., J. Biol. Chem. 275(5): 3687 (2000).

Additional Info

Recognizes human Sox-9 (~65kD). Species Crossreactivity: mouse, bovine.