Sox-9a (Sox9a, HMG Box Transcription Factor Sox9a, Transcription Factor Sox9a, Dr.80814)

SOX-9 is a member of the family of SOX (Sry-type high mobility group box) gene that were first identified on the basis of region with high homology to that of Sry (Sex-determining region Y). It is a transcription factor with a high mobility group DNA-binding domain that is expressed in all prechondrocytic and chondrocytic cells during embryonic development in a pattern that close parallels that of the gene for type II collagen. Phosphorylation of SOX-9 by PKA increases its DNA binding and transcriptional activity. SOX-9 regulates expression of chondrocyte phenotype related genes. IL-1 and TNF-a can cause a marked and rapid decrease in the levels of SOX-9 mRNA and/or protein in chondrocytes, thus strongly inhibit the expression of genes for cartilage extracellular matrix proteins. The down-regulation of SOX-9 may have a crucial role in inhibiting expression of the cartilage phenotype in inflammatory joint diseases.\n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:5000-1:20,000\nWestern Blot: 1:500-1:1000\nOptimal dilutions to be determined by the researcher.\n\nControl Peptide: S5364-28R\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S5364-28F

Size

250ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

ZF/Fish

Form

Supplied as a liquid in 40mM MOPS, pH 7.5, 0.1% BSA, 0.05% sodium azide, 50% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Wright EM et al (1993) Nucleic Acids Res. 21, 744. 2. Sekiya I et al (2000) J. Biol. Chem. 275 (15), 10738. 3. Crombrugghe B et al (2000) Matrix Biol 19(5), 389. 4. McDowall S et al (1999) J. Biol. Chem 274(34), 24023-24030. 5. Koopman SI et al (2001) J Endocrinol 169(3), 573-9. 6. Murakami S et al (2000) J Biol.Chem. 275(5): 3687.

Additional Info

Recognizes zebrafish SOX-9a.