Sp1 Transcription Factor

Sp1 is a member of the Sp (XKLF) family of transcription regulators, which are involved in the regulation of multiple genes. Sp1 has been implicated in a range of cellular processes including cell cycle regulation, maintenance of gene activation during development, regulation of nucleic acid metabolism and preservation of chromatin structure at gene loci. Sp1 binds to GC-rich motifs within DNA, and has been shown to interact with other tissue-specific transcription factors to modulate the activities of a variety of promoters. Sp1 is ubiquitously expressed in cell nuclei, although the level of expression may vary between cell types. \n\nApplications: \nSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:2500-1:10,000\nImmunoprecipitation: 1-4ug/mg of total protein\nOptimal dilutions to be determined by the researcher.\n\nRecommended Secondary Reagents:\nI1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb\nI1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb\nI1904-40J: IgG, Fc (HRP) Pab Gt xRb\nI1904-40P: IgG, Fc, X-Adsorbed (HRP) Pab Gt xRb\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S5369-95B

Size

100ug

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid with TRIS-citrate/phosphate, pH 7.5, 0.09% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Ammendola, R., Mesuraca, M., Russo, T., and Cimino, F. (1992). Sp1 DNA binding efficiency is highly reduced in nuclear extracts from aged rat tissues. The Journal of Biological Chemistry 267: 17944-17948.2. Courey, A.J., Holtzmann, D.A., Jackson, S.P., and Tjian, R. (1989). Synergistic activation by the glutamine-rich domains of human transcription factor Sp1. Cell 59: 827-836.3. Courey, A.J., and Tjian, R. (1988). Analysis of Sp1 in vivo reveals multiple transcriptional domains, including a novel glutamine-rich activation motif. Cell 55: 8878-8898.4. Jackson, S.P., MacDonald, J.J., Lees-Miller, S., and Tjian, R. (1990). GC Box binding induces phosphorylation of Sp1 by a DNA-dependent protein kinase. Cell. 63: 155-165.5. Jackson, S.P., and Tjian, R. (1988). O-glycosylation of eukaryotic transcription factors: implications for mechanisms of transcriptional regulation. Cell. 55.6. Kadonaga, J.T., Carner, K.R., Masiarz, F.R., and Tjian, R. (1987). Isolation of cDNA encoding transcription factor Sp1 and functional analysis of the DNA binding domain. Cell 51: 1079-1090.7. Kingsley, C., and Winoto, A. (1992). Cloning of GT box-binding proteins: A novel Sp1 multigene family regulating T-cell receptor gene expression. Molecular and Cellular Biology 12: 4251-4261.8. Macleod, D., Charlton, J. Mullins, J., and Bird, A.P. (1994). Sp1 sites in the mouse aprt gene promoter are required to prevent methylation of the CpG island. Genes & Development 8: 2282-2292.9. Majello, B., De Luca, P., Hagen, G., Suske, G., and Lania, L. (1994). Different members of the Sp1 multigene family exert opposite transcriptional regulation of the long terminal repeat of HIV-1. Nucleic Acids Research 22: 4914-4921.10. Perkins, N.D., Lawrence Edwards, N., Duckett, C.S., Agranoff, A.B., Schmid, R.M., and Nabel, G.J. (1993). A co-operative interaction between NK-kB and Sp1 is required for HIV-1 enhancer activation. EMBO Journal 12: 3551-3558.11. Saffer, J., Jackson, S.P., and Thurston, S.J. (1990). SV40 stimulates expression of the trans-acting factor Sp1 at the mRNA level. Genes & Development 4: 659-666.12. Saffer, J.D., Jackson, S.P., and Annarella, M.B. (1991). Developmental expression of Sp1 in the mouse. Molecular and Cellular Biology 11: 2189-2199.

Additional Info

Recognizes human transcription factor Sp1.