SP19 (pan Sodium Channel)

Applications:\nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution: \nWestern Blot: 1:200 using ECL on rat brain membranes\nImmunohistochemistry: Frozen mouse brain sections; Paraffin mouse spinal cord sections.\nNote: All procedures that are going to receive a full-length protein should be performed at 4 degrees C, and the following protease inhibitor mixture should be used: pepstatin A (1ug/ml), leupeptin (1ug/ml), aprotinin (1ug/ml), Pefabloc SC (0.2mM), benzamidine (0.1mg/ml), and calpain inhibitors I and II (8ug/ml each).\nOptimal dilutions to be determined by the researcher.\n\nIncluded Negative Control: \nS5368A: SP19 (pan Sodium Channel), Rat, Control Peptide\nSupplied as a lyophilized powder. Reconstitute in 100ul sterile ddH2O. Preincubate 1ug of peptide with 1ug of antibody for one hour at RT.\n\n*Recommended Secondary Antibodies\nI1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb\nI1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb\nI1904-46Q: IgG, H&L (HRP) Pab Gt xRb\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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