Specific Intracellular Adhesion Molecule Grabbing Nonintegrin R1, Low Endotoxin (SIGN-R1, CD209 antigen-like protein B)

Mouse SIGN-R1 is a C-type lectin that has been identified as a murine homologue of the human DC-SIGN related molecule, L-SIGN. Mouse SIGN-R1 is specifically expressed on marginal zone macrophages in the spleen, medullary and subcapsular sinus macrophages in the lymph nodes, and at lower levels on liver sinusoidal endothelial cells. SIGN-R1 has not been detected on murine dendritic cell populations.\n\nLike many C-type lectins, SIGN-R1 functions as a pathogen recognition receptor in vivo and plays an important role in defence against pathogens.\n\nApplications:\nSuitable for use in Flow Cytometry and Immunohistochemistry (frozen). Other applications not tested. \n\nRecommended Dilution:\nFlow Cytometry: 10ul labels 10e6 cells in 100ul\nImmunohistochemistry (frozen): 1:25-1:50\nOptimal dilutions to be determined by the researcher. \n\nHybridoma: \nP3-X63-Ag8.563 myeloma cells with spleen cells from immunized rats.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S1013-69C

Size

500ug

Applications

FC, IHC

Hosts

Rat

Reactivities

Mouse

Form

Supplied as a liquid in PBS, pH 7.4. No preservative added.

P Type

Mab

Purity

Purified by ammonium sulfate precipitation.

Isotype

IgM

References

1. van Vliet, E. et al. (1985) Marginal zone macrophages in the mouse spleen identified by a monoclonal antibody. J. Histochem Cytochem. 33: 40-44. 2. Teunis, B.H. et al. (2002) Marginal zone macrophages express a murine homologue of DC-SIGN that captures blood-borne antigens in vivo. Blood. 100: 2908-2916. 3. Kang, Y.S. et al. (2003) SIGN-R1, a novel C-type lectin expressed by marginal zone macrophages in spleen, mediates uptake of the polysaccharide dextran. Int Immunol. 15:177-186. 4. Kraal, G. et al. (1989) Marginal zone macrophages and their role in the immune response against T-independent type 2 antigens: modulation of the cells with specific antibody. Eur. J. Immunol. 19: 675-680.

Additional Info

Recognizes mouse SIGN-R1.