Specific Intracellular Adhesion Molecule Grabbing Nonintegrin R1 (SIGN-R1, CD209 antigen-like protein B)

SIGN-R1 is a specific marker for the identification of macrophage subpopulations present in the marginal zone of spleen (the so-called marginal zone macrophages (MZM)), in the lymph node medulla, and in some strains, in the peritoneal cavity. Mouse SIGN-R1 is a C-type lectin, like DC-SIGN which is expressed on human dendritic cells (DCs). However, mouse SIGN-R1 itself is not expressed on DCs. SIGN-R1 exists in an aggregated form, resistant to dissociation into monomers upon boiling in SDS under reducing conditions. SIGN-R1 mediates the uptake of encapsulated organisms and may be an important mediator for the uptake of microbes in both spleen and lymph node, particularly through the recognition of microbial polysaccharides. Uptake of FITC-labeled dextran by macrophages can be blocked both in vivo and in vitro by the monoclonal antibody. Therefore, the monoclonal antibody can be used to study the uptake of polysaccharides by macrophages. \n\nApplications:\nSuitable for use in Flow Cytometry, Functional Blocking Studies, and Immunohistochemistry. Other applications not tested. \n\nRecommended Dilution:\nFlow Cytometry: 1:50\nImmunohistochesmitry (frozen and paraffin): 1:50\nFor blocking studies in vitro, dilutions have to be made according to the amounts of SIGN-R1 to be inactivated. Optimal dilutions to be determined by the researcher.\n\nPositive Control: Peritoneal macrophages\n\nNegative Control: Alveolar macrophages\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S1013-69A

Size

1ml

Applications

FC, IHC

Hosts

Rat

Reactivities

Mouse

Form

Supplied as a liquid in 0.02% sodium azide. Low Endotoxin.

P Type

Mab

Purity

Supernatant

Isotype

IgM

References

1. Crowley, M et al; Influence of lymphocytes on the presence and organization of dendritic cell subsets in the spleen. J Immunol 1999, 163: 4894 2. Kang, Y et al; SIGN-R1, a novel C-type lectin expressed by marginal zone macrophages in spleen, mediates uptake of the polysaccharide dextran. Int Immunol 2003, 15: 177 3. Kang, Y et al; The C-type lectin SIGN-R1 mediates uptake of the capsular polysaccharide of Streptococcus pneumoniae in the marginal zone of mouse spleen. PNAS 2004, 101: 215 4. Taylor, P et al; The role of SIGN-R1 and the beta-glucan receptor (dectin-1) in the nonopsonic recognition of yeast by specific macrophages. J Immunol 2004, 172: 1157 5. Taylor, P et al; Development of a specific system for targeting protein to metallophilic macrophages. PNAS 2004, 101: 1963 6. Nagaoka, K et al; Association of SIGNR1 with TLR4-MD-2 enhances signal transduction by recognition of LPS in gram-negative bacteria. Int Immunol 2005, 17: 827

Additional Info

Recognizes murine SIGN-related 1 (SIGN-R1). Does not react with macrophages in other regions of the spleen, such as CD169+ marginal metallophils and F4/80+ red pulp macrophages. MZM of the spleen are involved in the clearance of polysaccharides.

Alternative Names

CD209 antigen-like protein B