Applications:
Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications have not been tested.
Recommended Dilutions:
Immunohistochemistry: Frozen 1:25-1:50.
Optimal dilutions to be determined by the researcher.
Hybridoma:
Hybridization of P3-X63-Ag8.563 myeloma cells with spleen cells from immunized rats.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.