Specific Intracellular Adhesion Molecule Grabbing Nonintegrin R1 (SIGN-R1)

Applications: \nSuitable for use in Flow Cytometry and Immunohistochemistry. Other applications have not been tested. \n\nRecommended Dilutions: \nImmunohistochemistry: Frozen 1:25-1:50. \nOptimal dilutions to be determined by the researcher. \n\nHybridoma:\nHybridization of P3-X63-Ag8.563 myeloma cells with spleen cells from immunized rats.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S1013-69

Size

250ug

Applications

FC, IHC

Hosts

Rat

Reactivities

Mouse

Form

Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide

P Type

Mab

Purity

Purified by ammonium sulfate precipitation.

Isotype

IgM

References

1. van Vliet, E., et al., (1985),

Additional Info

Recognizes mouse SIGN-R1, a C-type lectin that has been identified as a murine homolog of the human CD-SIGN related molecule, L-SIGN. Mouse SIGN-R1 is specifically expressed on marginal zone macrophages in the spleen, medullary and subcapsular sinus macrophages in the lymph nodes, and at lower levels on liver sinusoidal endothelial cells. SIGN-R1 has not been detected on murine dendritic cell populations. Blocks the uptake of neutral polysaccharides by macrophages.