Specific Intracellular Adhesion Molecule Grabbing Nonintegrin R1 (Specific ICAM-3 Grabbing Nonintegrin R1, SIGN-R1, CD209 antigen-like Protein B)

SIGN-R1 is a specific marker for the identification of macrophage subpopulations present in the marginal zone of spleen (the so-called marginal zone macrophages (MZM)), in the lymph node medulla, and in some strains, in the peritoneal cavity. Does not react with macrophages in other regions of the spleen, such as CD169+ marginal metallophils and F4/80+ red pulp macrophages. MZM of the spleen are involved in the clearance of polysaccharides. Mouse SIGN-R1 is a C-type lectin, like DC-SIGN which is expressed on human dendritic cells (DCs). However, mouse SIGN-R1 itself is not expressed on DCs. SIGN-R1 exists in an aggregated form, resistant to dissociation into monomers upon boiling in SDS under reducing conditions. SIGN-R1 mediates the uptake of encapsulated organisms and may be an important mediator for the uptake of microbes in both spleen and lymph node, particularly through the recognition of microbial polysaccharides. Uptake of FITC-labeled dextran by macrophages can be blocked both in vivo and in vitro by the monoclonal antibody. Therefore, the monoclonal antibody can be used to study the uptake of polysaccharides by macrophages.\n\nApplications:\nSuitable for use in Immunohistochemistry and Flow Cytometry. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: 1:50. \nImmunohistochemistry (frozen, paraffin): 1:50. \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S5370-90

Size

100ug

Applications

FC, IHC

Hosts

Rat

Form

Supplied as a liquid.

P Type

Mab

Purity

Purified

Isotype

IgM

References

1. Crowley, M et al; Influence of lymphocytes on the presence and organization of dendritic cell subsets in the spleen. J Immunol 1999, 163: 4894 2. Kang, Y et al; SIGN-R1, a novel C-type lectin expressed by marginal zone macrophages in spleen, mediates uptake of the polysaccharide dextran. Int Immunol 2003, 15: 177 3.

Additional Info

Recognizes murine SIGN-related 1 (SIGN-R1).