Spectrin, alpha II, SH3 domain, non-erythroid (Fodrin

This antibody was developed against the alpha II spectrin SH3 domain.(1) Specific marker for human, rat and mouse alpha II spectrin or fodrin.\n\nSource: Tissue culture supernatant\n\nApplications: Suitable for use in ELISA, Western Blot, Immunofluorescence, Immunohistochemistry (Frozen only). Other applications not tested.\n\nRecommended Dilution: Immunofluorescence: 1:100-1:500, Immunohistochemistry: 1:50-1:100; Optimal dilutions to be determined by the researcher.\n\nStorage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S5380-05

Size

100ul

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.

P Type

Mab

Purity

Purified by ammonium sulfate precipitation and dialyzed.

Isotype

IgG2b

References

1. Xu, J., et al., \"Monoclonal antibodies to alpha I spectrin Src homology domain 3 associate with macropinocytic vesicles in nonerythroid cells\", Brain Research, 898: 171-177 (2001).\n2. Xu, J., et al., \"Human spectrin SH3 domain binding protein I regulates macropinocytosis in NIH 3T3 cells\", J. Cell Science, 113: 3805-3814 (2000).\n3. De Matteis, M.A., et al., \"Spectrin tethers and mesh in the biosynthetic pathway\", J. Cell Science, 113: 2331-2343 (2000).\n4. Swanson, J.A., et al., \"Macropinosytosis Trends\", Cell Biol., 5: 424-427 (1995)\n5. Lamaze, C., et al., \"The emergence of clathrin-independent pinocytic pathway\", Curr. Opin. Cell Biol., 7: 573-580 (1995).\n6. Bennett, V., et al., \"The spectrin-based membrane skeleton and micron scale organization of the plasma membrane\", Annu. Rev. Cell Biol., 9: 27-66 (1993).

Additional Info

In cerebellar cell cultures, it labels axons and periphery of cell bodies consistent with localization of fodrin. Does not crossreact with alpha I (erythroid) spectrin SH3 domain.