Spectrin, alpha (Spectrin alpha Chain, Spectrin alpha Chain Brain, Spectrin Non-erythroid alpha Chain, Alpha Fodrin, Alpha II Spectrin, FLJ44613, Fodrin alpha Chain, Non-erythrocytic Spectrin alpha, Spectrin alpha Non-erythrocytic 1, SPTAN1, SPTA2)

Alpha Fodrin is a member of the spectrin family of widely distributed filamentous proteins that form a supporting and organizing scaffold for cell membranes (1). Non-erythroid alpha spectrin, also called Alpha Fodrin, is a major cortical cytoskeletal protein that is implicated in the establishment of specialized membrane-cytoskeletal domains in differentiating cells (2, 3). Cleavage of Alpha Fodrin accompanies apoptosis, induced by activation via the CD3/T cell receptor complex, ligation of the Fas (CD95) molecule and other Fas-expressing cells; it may also be induced by the treatment of cells with staurosporine, dexamethasone, or synthetic ceramide (4). The major products of cleavage are amino-terminal and carboxy-terminal fragments. Antibodies against Alpha Fodrin are observed in diseases characterized by chronic apoptosis (5, 6).\n\nApplications:\nSuitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:10,000-1:50,000\nImmunohistochemistry (Paraffin): 1:500-1:1000\nImmunocytochemistry: 1:250-1:500\nImmunoprecipitation: 1:10-1:100\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. \n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

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SPECIFICATIONS

Catalog Number

S5379-96G

Size

100ul

Applications

ICC, IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Rat

Form

Supplied as liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% BSA, 0.01% sodium azide, 40% glycerol.

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1. Leto TL, et al. Mol Cell Biol. 8(1):1-9, 1988, 2. Simonovic M, et al. J Biol Chem. 281(45):34333-40, 2006, 3. DH Giebelhaus, et al. The Journal of Cell Biology 105:843-853,1987, 4. Martin SJ, et al. J Biol Chem. 270(12):6425-8, 1995, 5. Ulbricht K U, et al. Autoimmunity Reviews 2(2):109-113,2003, 6. Kahaly, G. J., et al. Clinical & Experimental Immunology140:166-172(7), 2005.

Additional Info

Recognizes human Spectrin, alpha at 285, 150kD. Species Crossreactivity: rat.