Spectrin, beta1 (Spectrin beta 1, Beta I Spectrin, Beta Spectrin, HSpTB1, Spectrin beta Chain Erythrocyte, Spectrin beta Erythrocytic (Includes Spherocytosis Clinical Type I), SPTB, SPTB1)

Spectrin (Sp), the most abundant of the erythrocyte membrane skeleton proteins, helps these cells maintain their characteristic biconcave shape while remaining flexible and elastic. Erythrocyte Sp is a heterodimer composed of a 280kD alpha subunit and a 246kD beta subunit which associate in a side-to-side, antiparallel configuration to form a 100 nm rod-like structure. Sp in other tissues may be composed of distinct but homologous alpha and beta subunits, sometimes referred to as fodrin. A newly introduced nomenclature designates the Sp subunits of the erythrocyte as alpha-1 and beta-1, and the fodrin subunits as alpha-2 and beta-2. Alternatively spliced forms of each are designated as epsilon-1, epsilon-2, etc. (e.g. beta-1 epsilon-1).\n\nApplications: \nSuitable for use in Immunofluorescence and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S5380-12A

Size

100ul

Applications

IF, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, pH 7.2, 0.05% sodium azide.

P Type

Mab

Purity

Ascites

Isotype

IgG1

References

This product has been used in:Porter GA et al. Two populations of beta-spectrin in rat skeletal muscle. Cell Motil Cytoskeleton 37:7-19 (1997). Dmytrenko GM et al. Dystrophin in a membrane skeletal network: localization and comparison to other proteins. J Neurosci 13:547-58 (1993). Winkelmann JC & Forget BG Erythroid and nonerythroid spectrins. Blood 81:3173-85 (1993).

Additional Info

Detects spectrin from erythrocytes, brain and muscle cells. This antibody has been shown to specifically detect the two known alternatively spliced forms of spectrin, beta-1 epsilon-1, present in erythrocytes, and beta-1 epsilon-2, present in nerve and striated muscle cells. It does not crossreact with alpha-2 spectrin or either of the fodrin subunits. Species Crossreactivity: Crossreacts with Human, Rat and Mouse. Not yet tested in other species.