The protein encoded by this gene was identified by a two- hybrid screen using CD4 as the bait. It binds to the hydrophobic C-terminal aa of CD4 which are involved in repression of T cell activation. The interaction with CD4 is mediated by the noncatalytic alpha/beta hydrolase fold domain of this protein. It is thus proposed that this gene product modulates the stimulatory activity of CD4.
Source:
Recombinant protein corresponding to SPG21, fused to T7 tag at N-terminal expressed in E.coli.
Molecular Weight:
~35.4kD
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution:
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Aliquot to avoid repeated freezing and thawing and store at -70 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.