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Sphingosine Kinase 1 Inhibitor Screening Assay Kit

Cat no: 700430

Sphingosine Kinase 1 Inhibitor Screening Assay Kit

SPHK 1 and SPHK 2 phosphorylate D-erythro-sphingosine to yield S1P. S1P exhibits a broad spectrum of biological activities including cell proliferation, survival, migration, cytoskeletal organization, and morphogenesis. SPHK 1 is a potential therapeutic target for the control of cancer and inflammation. Cayman's SPHK 1 Inhibitor Screening Assay Kit provides a fluorescence-based method for screening SPHK 1 inhibitors.

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SPECIFICATIONS

Catalog Number

700430

Form

96 Well

P Type

Assay Kits|Ceramides/Sphingolipids

Weight

0

Storage Temp

-20

Shipping Temp

-20

Additional Info

Sphingosine kinase 1 (SPHK 1) and SPHK 2 phosphorylate D-erythro-sphingosine to yield sphingosine-1-phosphate (S1P). S1P exhibits a broad spectrum of biological activities including cell proliferation, survival, migration, cytoskeletal organization, and morphogenesis. SPHK 1 is a potential therapeutic target for the control of cancer and inflammation Cayman's SPHK 1 Inhibitor Screening Assay Kit provides a fluorescence-based method for screening SPHK 1 inhibitors. The procedure requires only two easy steps, both performed in the same microplate. In the first step, human recombinant SPHK 1 phosphorylates D-erythro-sphingosine C-18. In the second step, ADP is converted to hydrogen peroxide (H2O2) which then reacts with ADHP in the presence of peroxidase to yield the fluorescent product resorufin.

SUPPLIER INFO

Cayman Chemical Company

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